I had recently upgraded my lab with a Soxhlet extractor. These things are GREAT! I decided to try extracting bufotenine from Anadenanthera colubrina using my Soxhlet extractor. After reading about Jonathan Ottยs freebase bufotenine experiences, I was dying to try it for myself. So I did an extraction.
Bufotenin
Well-documented
Well-documented
Multiple authoritative sources agree on dosing and effects.
- 4 corroborating sources
- 1 ROA with full dose ladder
- duration data present
- 19 combo interactions documented
- toxicity data (PsychonautWiki, PubChem)
- classified Research-chemical
Well-documented
Multiple authoritative sources agree on dosing and effects.
- 4 corroborating sources
- 1 ROA with full dose ladder
- duration data present
- 19 combo interactions documented
- toxicity data (PsychonautWiki, PubChem)
- classified Research-chemical
Aliases: Jopo, Hataj, Niopo, Cohoba, Mapine
Summary
Bufotenin is a naturally occurring tryptamine found in Anadenanthera (Yopo/Cebil) seeds and in the parotoid glands of certain Bufo and Incilius toads. The Colorado River toad (Incilius alvarius) contains primarily 5-MeO-DMT, not bufotenin, and 'toad-licking' is extremely dangerous due to cardiotoxic bufadienolides in the venom. Pure bufotenin freebase vaporizes at approximately 160ยฐC; use low-temperature glass pipes or e-mesh devices to minimize harshness.
Perspectives
“Within a minute (from the start of the injection) I had a tight feeling in my chest and my face felt as if it had been jabbed by nettles and this lasted for about 6 minutes. I had fleeting nausea.”
Dose Information
| ROA | Light | Common | Strong | Heavy |
|---|---|---|---|---|
| Smoked | 5-20mg | 20-40mg | 40-60mg | 60mg+ |
| Insufflated | ~50-100 mg (seeds, pulverized) | - | - | - |
Light
Common
Strong
Heavy
Onset, Duration & After-effects
| ROA | Onset | Comeup | Peak | Offset | Total |
|---|---|---|---|---|---|
| Smoked | 0-36 sec | 0-36 sec | 1-5 min | 5-10 min | 6-16 min |
| Insufflated | 2-10 min | 5-15 min | 20-40 min | 30-60 min | 0.94-2.09 hrs |
| Sublingual | 5 min | 10-20 min | 35-40 min | 30-60 min | 1.33-2.08 hrs |
| Oral | 30-60 min | 30-60 min | 1-2 hrs | 1-2 hrs | 3-6 hrs |
Effect Profile
4 reportsScores (1–10) curated from multiple sources:
- Effect keyword matching from PsychonautWiki catalog
- Weighted by importance: core (×3), major (×2), minor (×1)
Psychedelic
7.0
Strong+
7/10
Strong body load, visuals, headspace, and auditory effects
Visual Intensity
×3
8
color enhancement
drifting
color shifting
environmental orbism
visual acuity enhancement
Headspace Depth
×3
8
conceptual thinking
recursion
emotion enhancement
thought loops
time distortion
Auditory Effects
×1
8
auditory distortion
auditory distortions
Body Load / Somatic Effects
×1
10
vasoconstriction
temperature regulation suppression
nausea
bodily pressures
stomach discomfort
Based on reports from:
Add missing routes to PsychonautWiki Journal
The PsychonautWiki Journal app already has Bufotenin, but its built-in entry only covers Smoked. Here are the other routes we have data for. You can't edit a built-in substance, but you can add a Custom Unit for any of these routes to log it.
⬇ Download import file
Import this file as custom substances
and you get "Bufotenin (Substance Search)" with every
route's full dose ladder and timeline, no typing. Works on iOS and
Android (v15+); the format is confirmed by the app's
developer. The manual values below work for older
versions.
Dose & duration (reference)
Bufotenin: routes the PsychonautWiki Journal app is missing Source: SubstanceSearch (substancesearch.com) Insufflated dose (seeds): threshold Varies (effects at low doses) ยท light ~50-100 mg (, pulverized) Sublingual dose (mg): threshold 10 Oral dose (mg): threshold 10 Insufflated duration: onset 2-10m ยท come-up 5-15m ยท peak 20-40m ยท offset 0.5-1h ยท total 0.94-2.09h ยท after-effects 0.5-1.5h Sublingual duration: onset 5m ยท come-up 10-20m ยท peak 35-40m ยท offset 0.5-1h ยท total 1.33-2.08h ยท after-effects 0.5-1.5h Oral duration: onset 0.5-1h ยท come-up 0.5-1h ยท peak 1-2h ยท offset 1-2h ยท total 3-6h ยท after-effects 2-6h Note: ranges are harm-reduction guidance, not a prescription. Start low, go slow, and test your substances.
Custom Units carry a single dose per route, so the full ranges live in the reference above. A route's suggested dose is the midpoint of its common range. Ranges are harm-reduction guidance, not a prescription.
Tolerance
Build-up
develops rapidly after a single use
Reset
7โ14 days for baseline
Tolerance Decay
Half tolerance
7d
Baseline
~14d
Rapid acute tolerance is typical of short-acting tryptamines; sensitivity partially returns within hours but cross-tolerance to other psychedelics can persist into the next day. Data quality primarily anecdotal.
Cross-Tolerances
Effects
Aggregated from 4 Erowid experience reports
Positive Effects 2
Euphoria
100.0%
70%
Color Enhancement
75.0%
70%
Adverse Effects 0
Combinations
Cross-Check Bufotenin with another substanceCommunity Trip Reports
Anecdotal first-person accounts from Reddit, Erowid, and Bluelight. Click a source to expand. Reports are harm-reduction context, not medical guidance. Tags summarise each author's own verdict — how reports are classified.
Erowid 4 reports 3 positive 1 neutral visit
I weighed out 15mg of sticky, brown bufotenin resin and placed it in the pipe, playing the flame from a lighter over the tiny bead of material. It vaporised easily and cleanly, the hot smoke pungent with indole and the fishy amine aroma characteristic of bufotenin.
[ Erowid Note: One should not attempt to perform alkaloid extractions without first having an understanding of safe handling procedures for the chemicals and equipment required.] We had read several reports of free base bufotenin being a pleasant experience. We decided to give it a go and see for ourselves.
After having a great experience with sublingual free base bufotenine, the following weekend we played around with smoking free base bufotenine. We smoked doses ranging from 10 mg all the way up to 15 mg. All three of us had positive experiences. The effects are different this way.
PiHKAL / TiHKAL
TiHKAL #19: 5-HO-DMT
“Within a minute (from the start of the injection) I had a tight feeling in my chest and my face felt as if it had been jabbed by nettles and this lasted for about 6 minutes. I had fleeting nausea.”
Bufotenin's nerd page
Pharmacology, chemical data, effect profiles, and more
→
Helpful Links
Report an issue or suggest an edit
Spotted incorrect or dangerous information, have a correction, a new study, or a useful addition for Bufotenin? Let us know. Reports are reviewed by moderators — this is not posted publicly.